Enzymatic cleaning solutions for contact lenses also contain proteases to remove proteinīuild-up. They are both proteases, meaning they break apart proteins. These two enzymes are extracted from pineapple and papaya, respectively. The two most common enzymes used in meat tenderizer are Bromelain and Papain. It is broken down by the motion and physical force of the blender.Ħ. How is the cell wall of plant cells broken down? A cell's DNA is usually protected from such enzymes (called DNases) by the nuclear membrane, but adding detergent destroys that membrane.ĥ. Why would a cell contain enzymes that destroy DNA? These enzymes are present in the cell cytoplasm (not the nucleus) to destroy the DNA of viruses that mayĮnter our cells and make us sick. This protects DNA from enzymes that can destroy it. How? Cooling slows down enzymatic reactions. Why is cold water better than warm water for extracting DNA?Ĭold water helps keep the DNA intact during the extraction process. Salty water helps the DNA precipitate (solidify and appear) when alcohol is added.Ĥ. The cell soup should be opaque, meaning that you can't see through it. To fix this, go back to the first step and add less water. If the blended cell soup is too watery, there won'tīe enough DNA to see. Many food sources of DNA, such as grapes, also contain a lot of water. Make sure that you started with enough DNA. The cold alcohol helps the DNA precipitate (solidify and appear) more quickly.
The cold water protects the DNA by slowing down enzymes that can break Using ice-cold water and ice-cold alcohol will increase your yield of DNA.
Or, try letting the test tube of pea mixture and alcohol sit for 30-60 minutes. The DNA will be stuck in the bottom layer. If the cell and nuclear membranes are still intact,
Make sure to let the detergent sit for at least five minutes. What can I do to increase my yield of DNA?Īllow more time for each step to complete. Even if your yield of DNA is low,Ĭlumps of DNA may be loosely attached to the bubbles.Ģ. Also, look very closely at the alcohol layer for tiny bubbles. Into the pea soup and slowly pull upward into the alcohol layer. Try to help the DNA rise to the top, alcohol layer. Your DNA may be lingering between the two layers of alcohol and pea soup.
0 kommentar(er)
